03-P125 The Krox20 autoregulatory loop in the developing hindbrain: New insights from a quantitative approach

have been examined with cellular markers, such as cell proliferation, cytoskeletal formation and apoptosis to understand the precise mechanism of palatal rugae morphogenesis. In particular, expression patterns of signalling molecules, Shh and Bmp4, which express in rugae and inter-rugae regions respectively, suggest that molecular interactions would govern the epithelial differentiation and pattern formation of palatal rugae. In this study, we employed in vitro organ culture method at E12 palate for 3 days to define the epithelial cell lineage during rugae morphogenesis. We also performed the gain and loss of function studies using protein soaked beads implantation and antisense-oligodeoxynucleotide treatment to understand the role of molecular interactions in morphogenesis. Overall, molecular interactions and cellular events with the specific pattern through the developmental process of rugae formation would be a plausible answer to understand the precise differentiation pathway of rugae epithelium.